Inhibition of the Notch1 Pathway Promotes the Effects of Nucleus Pulposus Cell-Derived Exosomes on the Differentiation of Mesenchymal Stem Cells into Nucleus Pulposus-Like Cells in Rats

Figure 2

Identification of NPC exosomes and internalization of NPC exosomes by MSCs. (a) Characteristics of exosomes derived from NPCs under a transmission electron microscope. (b) Western blot analyses of exosomal protein markers CD63, CD81, and Tsg101 and negative protein calnexin. CL: NPC cell lysate; EXO: NPC exosomes. (c, A) Internalization of NPC exosomes by MSCs. NPC exosomes were stained with CM-Dil (B), and MSCs were stained with CM-Dio (C). Internalization was observed by a fluorescence microscope (D).

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