Spontaneously Formed Spheroids from Mouse Compact Bone-Derived Cells Retain Highly Potent Stem Cells with Enhanced Differentiation Capability

Table 2

Quantitative reverse transcription-PCR primer set list.

Primer	Direction	Sequence (5-3)
β-Actin	Forward	CATCCGTAAAGACCTCTATGCCAAC
	Reverse	ATGGAGCCACCGATCCACA
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH)	Forward	GGTGTGAACCACGAGAAA
	Reverse	TGAAGTCGCAGGAGACAA
Sox2/sex determining region Y (SRY)-box 2	Forward	GTTCTAGTGGTACGTTAGGCGCTTC
	Reverse	TCGCCCGGAGTCTAGCTCTAAATA
Fucosyltransferase 4 (FUT4·SSEA1)	Forward	GCAGGGCCCAAGATTAACTGAC
	Reverse	AAGCGCCTGGGCCTAAGAA
Octamer-binding transcription factor 4 (Oct4)	Forward	CAGACCACCATCTGTCGCTTC
	Reverse	AGACTCCACCTCACACGGTTCTC
Nanog	Forward	TGCCAGTGATTTGGAGGTGAA
	Reverse	ATTTCACCTGGTGGAGTCACAGAG
Hypoxia-inducible factors 2α (HIF-2α)	Forward	CAGTACTCCCACAGGCCTGACTAAC
	Reverse	GACTGTCACACCGCTGCCATA
CD105	Forward	CTGCCAATGCTGTGCGTGAA
	Reverse	GCTGGAGTCGTAGGCCAAGT
CD44	Forward	CAAGCCACTCTGGGATTGGTC
	Reverse	GGCAAGCAATGTCCTACCACAAC
CD29	Forward	CCATGCCAGGGACTGACAGA
	Reverse	GAGCTTGATTCCAATGGTCCAGA
Stem cell antigen-1 (Sca-1)	Forward	TTGCCTTTATAGCCCCTGCT
	Reverse	GTCATGAGCAGCAATCCACA
Kruppel-like factor 4 (KLF4)	Forward	AACATGCCCGGACTTACAAA
	Reverse	TTCAAGGGAATCCTGGTCTTC
Transcription factor Sp7/osterix (OSX)	Forward	AGGCCTTTGCCAGTGCCTA
	Reverse	GCCAGATGGAAGCTGTGAAGA
Bone sialoprotein (BSP)	Forward	GAGACGGCGATAGTTCC
	Reverse	AGTGCCGCTAACTCAA
Dentin matrix protein 1 (DMP1)	Forward	AGTGAGTCATCAGAAGAAAGTCAAGC
	Reverse	CTATACTGGCCTCTGTCGTAGCC
Microtubule-associated protein 2 (MAP2)	Forward	CAGTTTGGCTGAAGGTAGCTGAA
	Reverse	CACATCTGTGTGAGTGTGTGTGGA
Nestin	Forward	GAGGTGTCAAGGTCCAGGATGTC
	Reverse	ACACCGTCTCTAGGGCAGTTACAA
Nerve growth factor receptor (NGFR)/P75NTR	Forward	TCTGATGGAGTCGGGCTAATGTC
	Reverse	CCACAAATGCCCTGTGGCTA
Neuronal differentiation (NeuroD)	Forward	CAAAGCCACGGATCAATCTTC
	Reverse	TGTACGCACAGTGGATTCGTTTC