Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture

Figure 2

Maintenance of mouse colon organoids in Matrigel and in collagen-based matrix culture system. (a) Growth pattern of mouse colon organoid during Passage 3 in both Matrigel and collagen. Bar scale is 50 μm. (b) Measured circumstance of mouse colon organoid in Passage 3. (c) In vitro growth of mouse colon organoids during Passage 5 in Matrigel and collagen matrix. (d) Organoid viability was measured using the WST assay. The organoids in Matrigel were used as a control. (e) Quantitative RT-PCR was performed to measure the expression of specific markers of colon organoids at Passage 4 cultured in both matrices (left). Muc2 (mucin 2) and Lyz (lysozyme) were used as markers of goblet cells and Paneth cells, respectively. Gene expression shown as gel electroporation (right). Day and Passage denote days of in vitro and passage number, respectively. Scale bar: 50 μm. (f) The immunocytochemistry staining of Ki67, mucin 2, and chromogranin A was examined in Matrigel and Collagen matrix. Scale bar: 100 μm.

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