Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture

Figure 6

Transplantation of collagen matrix–cultured colon organoids into recipient mouse colonic epithelial. (a) C57BL/6 the recipient tissues were analyzed 7 days after transplantation. EGFP+ engraftments were present in both culture matrices (Matrigel and collagen). (b) Sections of the engrafted tissue 1 week posttransplantation show the presence of EGFP+ crypt cells, Ki67, villin, and chromogranin A differentiated cells in the crypts of donor origin, with Hoechst 33342 (nucleus) staining. Scale bar: 100 μm. (c) Colonic lesion visual analysis of recipient mouse colon. Red box: engraftment site.

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