Development of Collagen-Based 3D Matrix for Gastrointestinal Tract-Derived Organoid Culture

Table 2

PCR primer.

Species	Gene name	Direction	Sequence
Mouse	GAPDH	Forward	AACTTTGGCATTGTGGAAGG
		Reverse	ACACATTGGGGGTAGGAACA
Mouse	E-cadherin	Forward	ACCACTGCCCTCGTAATCGAA
		Reverse	CGTCCTGCCAATCCTGATGAA
Mouse	Lgr5	Forward	GACGCTGGGTTATTTCAAGTTCAA
		Reverse	CAGCCAGCTACCAAATAGGTGCTC
Mouse	Mucin-2	Forward	ATGCCCACCTCCTCAAAGAC
		Reverse	GTAGTTTCCGTTGGAACAGTGAA
Mouse	Lysozyme	Forward	GAGACCGAAGCACCGACTATG
		Reverse	CGGTTTTGACATTGTGTTCGC
Mouse	Defensin-5	Forward	AGGCTGATCCTATCCACAAAACAG
		Reverse	TGAAGAGCAGACCCTTCTTGGC
Mouse	Villin-1	Forward	GACGTTTTCACTGCCAATACCA
		Reverse	CCCAAGGCCCTAGTGAAGTCTT
Mouse	Chromogranin A	Forward	AAGGTGATGAAGTGCGTCCT
		Reverse	GGTGTCGCAGGATAGAGAGG
Human	GAPDH	Forward	GGTATCGTGGAAGGACTCATGAC
		Reverse	ATGCCAGTGAGCTTCCCGTTCAG
Human	Lgr5	Forward	TATGCCTTTGGAAACCTCTC
		Reverse	CACCATTCAGAGTCAGTGTT
Human	Mucin-5AC	Forward	TGATCATCCAGCAGCAGGGCT
		Reverse	CCGAGCTCAGAGGACATATGGG
Human	Mucin-2	Forward	CTGCACCAAGACCGTCCTCATG
		Reverse	GCAAGGACTGAACAAAGACTCAGAC
Human	Villin-1	Forward	GCAGCATTACCTGCTCTACGTT
		Reverse	GCTTGATAAGCTGATGCTGTAATTT
Human	Chromogranin B	Forward	CAGCCAACGCTGCTTCTC
		Reverse	TGGCATGGAATTGACAGC
Human	Lysozyme	Forward	CCGCTACTGGTGTAATGATGG
		Reverse	CATCAGCGATGTTATCTTGCAG
Mouse	Gastric intrinsic factor	Forward	TGAATCCTCGGCCTTCTATG
		Reverse	CAGTTAAAGTTGGTGGCACTTC
Mouse	Pepsinogen	Forward	CCAACCTGTGGGTGTCTTCT
		Reverse	TTAGGGACCTGGATGCTTTG
Mouse	Mucin 5AC	Forward	CCATGAAGTGGGAGTGTGTG
		Reverse	TTGGGATAGCATCCTTCCAG
Mouse	Mucin 6	Forward	TGCATGCTCAATGGTATGGT
		Reverse	TGTGGGCTCTGGAGAAGAGT
Mouse	Ghrelin	Forward	GCCCAGCAGAGAAAGGAATCCA
		Reverse	GCGCCTCTTTGACCTCTTCC
Mouse	Somatostatin	Forward	GAGGCAAGGAAGATGCTGTC
		Reverse	GGGCATCATTCTCTGTCTGG