Identification of WNT16 as a Predictable Biomarker for Accelerated Osteogenic Differentiation of Tonsil-Derived Mesenchymal Stem Cells In Vitro

Figure 1

Generation of monoclonal T-MSC subpopulations. (a) Osteogenic (OB) and adipogenic (AD) differentiation was induced in T-MSCs from four different donors. Matrix mineralization and lipid droplet formation were examined by Alizarin red S and oil red O staining, respectively, under phase-contrast microscopy (100x magnification for OB and 200x magnification for AD). (b) Surface marker expression in T-MSCs selected for further single-cell cloning was examined by flow cytometry. (c) OB differentiation was induced in monoclonal cells, and Alizarin red S staining was used to assess matrix mineralization. Representative images of six nondifferentiating (ND) and six osteoblast-prone (OP) clones were shown (100x magnification).

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