Downregulation of an Evolutionary Young miR-1290 in an iPSC-Derived Neural Stem Cell Model of Autism Spectrum Disorder

Figure 1

Neural progenitor markers are significantly enhanced in ASD-NSCs. (a) Immunostaining and statistical analysis indicate significantly more progenitor cells (Pax6⁺, Nestin⁺, and Sox2⁺) in ASD-NSCs than in control-NSCs. Bar = 20 μm. , , and (), determined by an unpaired -test and corrected by the Holm-Sidak multiple correction post hoc test. (b) No differences in Nestin⁺ or Sox2⁺ NSCs were observed in day 7-differentiated control and ASD-NSCs; however, significantly more progenitor cells were observed in day 21-differentiated ASD-NSCs. (), determined by an unpaired -test and corrected by the Holm-Sidak multiple correction post hoc test. Bar = 20 μm. Data are represented as the .

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