MicroRNA-28-5p Regulates Liver Cancer Stem Cell Expansion via IGF-1 Pathway

Figure 1

miR-28-5p is downregulated in liver CSCs. (a) EpCAM-positive primary HCC cells and their negative control HCC cells were sorted by flow cytometry and then subjected to RT-PCR assay (). (b) CD24-positive primary HCC cells and their negative control HCC cells were sorted by flow cytometry and then subjected to RT-PCR assay (). (c) Real-time PCR analysis of miR-28-5p in EpCAM-positive HCC cells and EpCAM-negative HCC cells (). (d) Real-time PCR analysis of miR-28-5p in CD24-positive HCC cells and CD24-negative HCC cells (). (e) The expression of miR-28-5p in primary HCC spheroid cells and primary HCC adherent cells was determined by RT-PCR assay (). (f) The expression of miR-28-5p in in HCC spheroid cells and HCC adherent cells was determined by RT-PCR assay (). (g) The expression of miR-28-5p in attached cells, spheroids, and reattached hepatoma cells was determined by RT-PCR assay (). (h) The correlation between the transcription level of miR-28-5p and EpCAM in forty HCC tissues was determined by RT-PCR analysis. Data were normalized to U6 or β-actin as ΔCt and analyzed by Spearman’s correlation analysis. (i) The correlation between the transcription level of miR-28-5p and CD24 in forty HCC tissues was determined by RT-PCR analysis. Data were normalized to U6 or β-actin as ΔCt and analyzed by Spearman’s correlation analysis. Data are represented as ; ; two-tailed Student’s -test.

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