Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid

Figure 1

Lamination of human fetal retina and Islet1 dynamic expression in human fetal retina and hiPSC-derived retinal organoid. (a–f) Ganglion cell layer (GCL) first developed in the human fetal retina. Next, the neuroblast layer gradually thickened and divided into an inner nucleus layer (INL) and outer nucleus layer (ONL). (a–f) Islet1 was expressed in the GCL in the early stage and then appeared as a monolayer nucleus on the most outer side. Later, sporadic positive nuclei were observed in the thickening neuroblast layer and finally collected on the outer side of the INL. (a–f) Consistent with the human fetal retina, Islet1 was expressed in the GCL first in the retinal organoid. A monolayer of Islet1-positive cells was located on the apical side. In the midperiod, rosettes were detected in the organoid, disturbing lamination. Until late stages, retinal organoid was laminated and Islet1-positive cells were collected in the INL.