Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid

<div>Islet1 coexpressed with Brn3 in retinal ganglion cells. (a–f) Islet1 and Brn3 colabeled retinal ganglion cells in the human fetal retina. From Fwk 20, Brn3 expression gradually decreased and was negative in Fwk 27. (g–i) Islet1-/Brn3-positive retinal ganglion cells were located on the basal side of the retinal organoid but were not laminated well. (j–l) Only sporadic Islet1-positive cells were on the basal side and were Brn3 negative. (m) Most Brn3-positive retinal ganglion cells exited the cell cycle in Fwk 9 retina. The high magnification showed one cell with Brn3 expression but still in the cell cycle. (n1) Triple staining with Islet1, Brn3, and progenitor marker MCM2 in Fwk 9 retina. 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Stem Cells International

fig2

Figure 2

Figure 2: Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid 