Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid

Figure 4

Colabeling RGC with RBPMS, ISL, and Brn3 in human fetal retina, retinal organoid, and rhesus retina. (a) Colabeling RGC with RBPMS and Islet1 in fetal retina. (b) Costaining fetal retina with RBPMS and Brn3. (c) Double staining of HuD and RBPMS in fetal retina. (d) RPBMS expression shared the same pattern in retinal organoids. (e1, e2) In the human adult retina, Islet1 was expressed in retinal ganglion cells (white triangle) but these cells were Brn3 negative. (f1, f2) Human adult ganglion cells were HuD positive (white triangle) but Brn3 negative. (g1) No Brn3-positive cells were detected in rhesus retina. (g2) Retinal ganglion cells were RPBMS positive but could not been stained by Brn3 antibody. (g3) HuD antibody stained retinal ganglion cells along with RBPMS. (g4) Islet1 was positive in both GCL and INL, but RPBMS only labeled retinal ganglion cells. (h) Brn3 immunofluorescence intensity on human fetal retina, along with RBPMS. .