Islet1 and Brn3 Expression Pattern Study in Human Retina and hiPSC-Derived Retinal Organoid

Figure 8

Isl1 expression in developing photoreceptor cells. (a–d) A monolayer of Islet1 immunoreactive nuclei was observed from Fwk 15; the cells were Recoverin positive. However, as indicated by the white triangle, some Recoverin-positive cells were Islet1 negative, and their nuclei were located on the inner side to Islet1-positive cells. (e) In Dwk 15, Recoverin-positive developing photoreceptors were migrating from the basal side to the apical side. (f) Similar to in the fetal retina, some developing photoreceptors with inner arranged nuclei were Recoverin positive but Islet1 negative. (g–h) Developing photoreceptors in the retinal organoid. (i) In Fwk 27, cells with inner arranged nuclei were Rhodopsin-positive rods, and the Islet1-nuclei were between the soma and inner segment of rods as the schematic diagram (h). (j–m) Rod differentiation in retinal organoids ().