Static Magnetic Field Induced Neural Stem/Progenitor Cell Early Differentiation and Promotes Maturation

Figure 5

Electrophysiological characterization of the mNPC-derived neuron. (a) Representative images showing the Tuj1 staining for the neurite outgrowth of mNPC-derived neurons. SMF stimulation (right panel) markedly increased the length and the branching of neurites than control neuron (left panel). Scale bar, 25 μm. (b) Responses of the mNPC-derived neuron to current injections (-60, -30, 0, +30 pA, duration, 500 ms, holding potential, -60 mV). (c) Representative current-clamp recordings of the mNPC-derived neuron. After exposure to SMF, mNPC-derived neuron exhibited spontaneous repetitive AP firing. (d) Representative examples of spontaneous postsynaptic currents at 3 weeks after differentiation (recordings were taken without blockers of synaptic transmission). Both control and SMF groups are showing spontaneous postsynaptic currents. (e) mNPC-derived neuron displayed a significantly increased in but not RMP or Cap after exposure to SMF. ; Student’s -test.

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