Improved Efficiency of Cardiomyocyte-Like Cell Differentiation from Rat Adipose Tissue-Derived Mesenchymal Stem Cells with a Directed Differentiation Protocol

Figure 2

Undifferentiated ASCs do not express cTnT, a specific cardiomyocyte marker. Flow cytometry analysis of cTnT of undifferentiated ASCs. Histogram overlay showing isotype control goat IgG-CFL-647 (black line) and cTnT-CFL 647 (blue). Percentage of cTnT-positive cells by flow cytometry; results are shown as ASCs (). Flow cytometry analysis of cTnT was performed in a pool of neonatal cardiomyocytes (isolation of 10 neonatal rat hearts); histogram overlay showing isotype control goat IgG-CFL-647 (black line) and cTnT-CFL 647 (blue). Flow cytometry for cTnT. (b) Undifferentiated ASCs do not express specific cardiomyocyte markers. Undifferentiated ASC and rat neonatal cardiomyocyte (positive control) immunostaining for Gata-4, cTnT, MyH, and SerCa2, each one with its isotype control (negative control); image obtained by confocal microscopy (scale bars, 50 μm).

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