IL-10 Gene-Modified Human Amniotic Mesenchymal Stem Cells Augment Regenerative Wound Healing by Multiple Synergistic Effects

Figure 9

Effects of hAMSCs on wound ECM remodeling on days 7 and 14 after cell transplantation. (a) TGF-β1 expression by groups at indicated time points. The hAMSCs-IL-10 group showed the highest expression of TGF-β1 on day 7 compared with the hAMSCs, hAMSCs-Null, and control groups. On day 14, expression of TGF-β1 in the control group was significantly higher than that in the other groups with the hAMSCs-IL-10 group showing the lowest expression. (b) MMP-1 expression by groups at indicated time points. The hAMSCs-IL-10 group showed the lowest expression of MMP-1 on day 7 compared with the hAMSCs, hAMSCs-Null, and control groups. On day 14, expression of MMP-1 in the hAMSCs-IL-10 group was significantly higher than that in other groups. (c) TIMP-1 expression by groups at indicated time points. Expression of TIMP-1 was significantly increased at days 7 and 14 in wounds treated with hAMSCs-IL-10, hAMSCs, or hAMSCs-Null compared with the control group; the hAMSCs-IL-10 group showed the highest level. (d) TIMP-1/MMP-1 ratio was highest in the hAMSCs-IL-10 group on day 7 and lowest on day 14. and ^&.

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