Does the Harvesting Site Influence the Osteogenic Potential of Mesenchymal Stem Cells?

Figure 4

Osteogenic differentiation determined by Alizarin Red S coloration. Confluent cells were cultured and induced in osteogenic medium (OM) or OM supplemented with 50 pg/ml IL-1β (IL-1β) up to 21 days. The control (CTR) cells were maintained in complete medium. Alizarin Red S solution was used to recognize calcium deposits. Quantification was performed by measuring the absorbance of the stained deposits at 450 nm wavelength. The lowest charts show the comparison in quantification among the cells originated from all three sources in both OM and IL-1β conditions. Data are the value of at least six independent experiments carried out in triplicate from at least six different cell cultures (, , ).