Efficient One-Step Induction of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs) Produces MSC-Derived Neurospheres (MSC-NS) with Unique Transcriptional Profile and Enhanced Neurogenic and Angiogenic Secretomes

Figure 3

Self-renewal and multipotency of UC-MSC-derived neurospheres. (a) Secondary neurosphere formation 24 hours after the dissociation of primary UC-MSC-derived neurospheres, phase contrast image. D2 denotes the differentiation of a UC-MSC-derived secondary neurosphere at day 2 in 2% fetal bovine serum (FBS) differentiation medium on poly-L-lysine and laminin double-coated coverslips. D7 denotes a fully differentiated secondary neurosphere at Day 7 after induction in 2% FBS differentiation medium on poly-L-lysine and laminin double-coated coverslips. (b and c) UC-MSC-derived secondary neurospheres were induced to differentiate into neurons and glial cells using 2% fetal bovine serum induction on poly-L-lysine and laminin double-coated coverslips. Confocal microscopic images of β-tubulin III (b) and GFAP (glial fibrillary acidic protein) immunohistochemistry staining (c) of differentiated UC-MSC-derived secondary neurospheres at day 7 after induction are shown. Dapi represents nucleic staining. .