Efficient One-Step Induction of Human Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSCs) Produces MSC-Derived Neurospheres (MSC-NS) with Unique Transcriptional Profile and Enhanced Neurogenic and Angiogenic Secretomes

Table 2

RayBio® human growth factor array. These readouts are the readouts after subtracting the background. Signal strength higher than 150-200 is considered significant. Expression level fold change larger than 1.5 is considered statistically significant.

Cytokines	UC-MSCs—D0	UC-MSCs—D1	UC-MSCs—D3	UC-MSCs—D5	UC-MSCs—D7
AR	360	942	16,610	3,677	17,070
BNDF	8,102	1,229	1,574	1,189	1,272
bFGF	4,602	41,624	31,735	40,812	34,468
BMP-4	934	1,320	1,179	1,228	1,502
BMP-5	692	760	790	696	844
BMP-7	554	723	777	779	737
b-NGF	580	398	471	418	427
EGF	1,659	33,719	47,861	52,847	54,487
EGF R	2,312	5,724	7,688	8,445	4,320
EG-VEGF	218	270	210	201	200
FGF-4	484	718	647	710	652
FGF-7	944	653	1,499	1,014	881
GDF-15	106,615	78,066	128,010	96,329	117,411
GDNF	1,088	916	9,405	1,627	6,965
GH	465	664	583	725	761
HB-EGF	264	498	486	521	795
HGF	9,735	13,519	97,528	32,492	86,731
IGFBP-1	223	578	478	622	636
IGFBP-2	255	679	1,952	1,161	1,930
IGFBP-3	1,898	422	322	397	433
IGFBP-4	2,085	609	2,156	671	892
IGFBP-6	8,868	5,660	7,395	4,292	2,215
IGF-1	5,067	5,372	5,386	6,033	5,988
Insulin	264	3,063	10,150	9,133	10,625
MCSF R	273	514	502	523	536
NGF R	390	703	724	704	659
NT-3	282	489	497	526	510
NT-4	459	663	551	595	550
OPG	1,569	6,750	28,573	22,742	28,604
PDGF-AA	2,167	2,537	2,628	1,165	1,464
PIGF	119	307	309	281	333
SCF	257	474	448	462	378
SCF R	1,000	707	2,247	728	1,533
TGF-α	0	149	207	180	204
TGF-β1	2,588	1,455	1,504	1,546	1,638
TGF-β3	118	345	240	292	248
VEGF	88	32,958	49,306	47,515	47,193
VEGF R2	529	775	677	550	705
VEGF R3	360	480	522	699	571
VEGF-D	178	429	627	664	785