Expression of Potential Dermal Progenitor Cell Markers in the Tumour and Stroma of Skin Adnexal Malignant and Benign Tumours

Figure 2

Herovici’s stain of skin appendage tumours: stain of young (blue) and mature (pink) collagen (Herovici’s stain), was used to allocate tumour stroma of skin appendage tumours as indicated by young collagen. The amount of cells that are present at either the invasive margin (IM) of the tumour, the inflammatory infiltrate surrounding the tumour (IIF), or the tumour-stroma interaction (TSI) site within the core of the tumour were determined for each tumour. Nodular basal cell carcinoma with stroma (blue indicating young collagen) within the tumour’s stroma interface and at the tumour’s invasive margin surrounded by its inflammatory interface (a, b). Adenoid basal cell carcinoma with blue stroma within the tumour surrounded by pink, mature collagen (c). Different stains of sclerosing basal carcinoma: blue young collagen largely present (d, f), almost absent (e) within the tumour and indicating the tumour margins (d) or invasive front (f) where tumour cells invade mature, pink collagen. Poroma with only mature (pink) collagen (g) in contrast to early porocarcinoma (h, l) with mixed blue and pink stroma and a margin of blue and pink (black arrow) and progressive porocarcinoma (l) with dominance of blue collagen with single fibers of pink collagen. Sebaceous carcinoma with tumour stroma as indicated by blue stained collagen ((i) and (m) with higher magnification).