Laminin as a Potent Substrate for Large-Scale Expansion of Human Induced Pluripotent Stem Cells in a Closed Cell Expansion System

Table 1

List of primers for pluripotency and germ layer differentiation.

	Primer	Forward	Reverse
Pluripotency	OCT4	CCATGCATTCAAACTGAGGTG	CCTTTGTGTTCCCAATTCCTTC
	NANOG	CTCCAGGATTTTAACGTTCTGC	TGGGATAAAGTGAGTTGCCTG
	LIN28	AAGAAATCCACAGCCCTACC	CCCCCCTAACCCATCACCTCCACCACCTAA
	SOX2	GAGAAGTTTGAGCCCCAGG	AGAGGCAAACTGGAATCAGG
	REX1	CAGATCCTAAACAGCTCGCAGAAT	GCGTACGCAAATTAAAGTCCAGA
	DPPA4	CAGCTCTGCTCATGACTGTTG	ATAGTAGCTAGCTTTGATGGCA
	NODAL	GGGCAAGAGGCACCGTCGACATCA	GGGACTCGGTGGGGCTGGTAACGTTTC
	TDGFb	CTGCTGCCTGAATGGGGGAACCTGC	GCCACGAGGTGCTCATCCATCACAAGG
	TERT3	CCTGCTCAAGCTGACTCGACACCGTG	GGAAAAGCTGGCCCTGGGGTGGAGC
	GDF3	GTGCCAACCCAGGTCCGGAAGTT	CTTATGCTACGTAAAGGAGCTGGG
Ectoderm	NESTIN	CACCTCAAGATGTCCCTCAG	AGCAAAGATCCAAGACGCC
	TUBB3	GCTCAGGGGCCTTTGGACATCTCTT	TTTTCACACTCCTTCCGCACCACATC
	NEFH	ACCTATACCCGAATGCCTTCTT	AGAAGCACTTGGTTTTATTGCAC
Mesoderm	MSX1	CGAGAGGACCCCGTGGATGCAGAG	GGCGGCCATCTTCAGCTTCTCCAG
	BMP4	GCACTGGTCTTGAGTATCCTG	TGCTGAGGTTAAAGAGGAAACG
	Brachyury (T)	GCCCTCTCCCTCCCCTCCACGCACAG	CGGCGCCGTTGCTCACAGACCACAGG
Endoderm	GATA6	CCAACTGTCACACCACAAC	TGGGGGAAGTATTTTTGCTG
	AFP	GAATGCTGCAAACTGACCACGCTGGAAC	TGGCATTCAAGAGGGTTTTCAGTCTGGA
	SOX17	GACGACCAGAGCCAGACC	CGCCTCGCCCTTCACC
	GAPDH	AATCCCATCACCATCTTCCAG	AAATGAGCCCCAGCCTTC