The Late Osteoblast/Preosteocyte Cell Line MLO-A5 Displays Mesenchymal Lineage Plasticity In Vitro and In Vivo

Figure 2

(a) In vitro mineral deposition by MLO-A5 determined by Alizarin Red staining and quantification, as described in Materials and Methods. Data shown are means of experimental triplicates () ± SEM; significant changes in mineralisation level are indicated by . (b) Real-time RT-PCR analysis of Col1a1, Tnap, Dmp1, Ocn, E11, and Phex gene expression in MLO-A5 cultures under osteogenic conditions. Data are shown as mean of biological triplicates assayed in duplicate from the day 0 value for each gene, except for Dmp1 which was normalised to the day 3 value due to undetectable levels at day 0. Significant changes are indicated by , , and .

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