The Late Osteoblast/Preosteocyte Cell Line MLO-A5 Displays Mesenchymal Lineage Plasticity In Vitro and In Vivo

Figure 4

(a) In vitro chondrogenesis by MLO-A5 in pellet cultures, as described in Materials and Methods, visualised by safranin O staining and alcian blue staining at pH 2.5. (b) GAG synthesis in MLO-A5 cells cultured under chondrogenic conditions in the presence or absence of recombinant TGF-β1. Data shown are of readings from quadruplicate wells; significance is indicated by . (c) Chondrogenic gene expression determined by real-time RT-PCR analysis of Sox9 and Col2a1 as well as the Col1a1/Col2a1 ratio. Data are shown as mean of biological triplicates assayed in duplicate from the day 3 value for each gene. Significant changes are indicated by .

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