The Late Osteoblast/Preosteocyte Cell Line MLO-A5 Displays Mesenchymal Lineage Plasticity In Vitro and In Vivo

Figure 5

Histological analysis of woven bone-like subcutaneous MLO-A5 tumour grafts: (a) H&E staining, with osteocyte lacunae evident (arrows); (b) alcian blue staining at pH 2.5; (c) safranin O staining; (d) immunostaining of small and large T antigens indicative of MLO-A5 origin; and (e) immunostaining with IgG negative control antibody. All images are of an identical magnification with the scale bar representing 40 μm and are representative of tissue from 3 mice.

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