A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation

<div>MSCs spontaneously form spheroids in TeSR-E8 medium on tissue culture dishes. (a) Phase contrast images showing the time-course of ADSC spheroid formation over 4 days. Scale bar: 150 <i>μ</i>m. (b) Size distribution of ADSC spheroids on day 4 postculture. (c, d) Morphology of monolayer and spheroid BMSCs and DPSCs. Scale bar: 150 <i>μ</i>m.</div>

Stem Cells International

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Figure 1

Figure 1: A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation 