A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation

<div>The viability and proliferative capacity of ADSC spheroids. (a) Fluorescence microscopic images of live/dead staining on day 4 postculture. Live cells within spheroids were stained with calcein AM (green), and dead cells were stained with PI (red). Scale bar: 100 <i>μ</i>m. (b) Morphology of ADSC spheroids after replacing TeSR-E8 with serum-containing medium. Scale bar: 150 <i>μ</i>m. (c) Cell cycle analysis via flow cytometry of monolayer ADSCs and ADSC spheroids. (d) Representative immunostaining of proliferation marker Ki67 in spheroid and monolayer ADSC after 4 days of culture. All nuclei were stained with DAPI (blue). Scale bar: 150 <i>μ</i>m. (e) Cell proliferation analysis using the CCK-8 assay. 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Stem Cells International

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Figure 3

Figure 3: A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation 