A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation

Figure 6

Therapeutic potential of ADSC spheroids in a mouse model of LPS-induced endotoxemia. (a) Survival rates of mice with LPS-induced endotoxemia following monolayer ADSCs and ADSC spheroid treatment within 48 h. Administration dosage of LPS was 25 mg/kg ( mice/group) and 50 mg/kg ( mice/group). (b) Cytokine levels were determined by ELISA in protein extracts from blood serum collected at 6 hours after LPS injection (50 mg/kg, -5 mice/group). vs. the control group. (c) Gating strategy for identification of Tregs with flow cytometry. (d) The percentages of CD4⁺CD25⁺Foxp3⁺ cells in the CD4 population of splenocytes isolated at 6 h after LPS injection (50 mg/kg, mice/group). (e) Gating strategy for identification of macrophages with flow cytometry. (f, g) The percentages of CD86⁺ (M1 macrophage biomarker) or CD206⁺ (M2 macrophage biomarker) cells in CD11b⁺F4/80⁺ population (total macrophages) of splenocytes isolated at 6 h after LPS injection (50 mg/kg, mice/group). vs. the monolayer ADSC group.

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