A Chemically Defined Serum-Free Culture System for Spontaneous Human Mesenchymal Stem Cell Spheroid Formation

Table 1

Primers used in real-time polymerase chain reaction gene expression analysis.

Gene	Primer sequence
RPS18	Forward	GCGGCGGAAAATAGCCTTTG
	Reverse	GATCACACGTTCCACCTCATC
Oct 4	Forward	CTTGAATCCCGAATGGAAAGGG
	Reverse	GTGTATATCCCAGGGTGATCCTC
Sox 2	Forward	CATGAAGGAGCACCCGGATT
	Reverse	GTTCATGTGCGCGTAACTGT
Nanog	Forward	AATGGTGTGACGCAGGGATG
	Reverse	TGCACCAGGTCTGAGTGTTC
LPL	Forward	TCATTCCCGGAGTAGCAGAGT
	Reverse	GGCCACAAGTTTTGGCACC
ALP	Forward	AACATCAGGGACATTGACGTG
	Reverse	GTATCTCGGTTTGAAGCTCTTCC
Runx2	Forward	TGGTTACTGTCATGGCGGGTA
	Reverse	TCTCAGATCGTTGAACCTTGCTA
Arg-1	Forward	CTCCAAGCCAAAGTCCTTAGAG
	Reverse	AGGAGCTGTCATTAGGGACATC
IL-10	Forward	CTTACTGACTGGCATGAGGATCA
	Reverse	GCAGCTCTAGGAGCATGTGG