Research Article
Bone Defect Repair Using a Bone Substitute Supported by Mesenchymal Stem Cells Derived from the Umbilical Cord
| | Gene symbol | Gene name | NCBI reference gene | Primer sequence | Product length | Product efficiency (%) |
| | GAPDH | Glyceraldehyde-3-phosphate dehydrogenase | NM_001289745.2 | F: GGTGTGAACCATGAGAAGTATGA | 123 | 1.91 | | R: GAGTCCTTCCACGATACCAAAG | | B2M | Beta-2-microglobulin | XM_005254549.3 | F: CCAGCGTACTCCAAAGATTCA | 94 | 1.85 | | R: TGGATGAAACCCAGACACATAG | | RPL13A | Ribosomal protein L13a | NM_001270491.1 | F: CGAGAAGAACGTGGAGAAGAAA | 105 | 1.92 | | R: GGCAACGCATGAGGAATTAAC | | HPRT1 | Hypoxanthine phosphoribosyltransferase 1 | NM_000194.2 | F: CGAGATGTGATGAAGGAGATGG | 98 | 2.22 | | R: TTGATGTAATCCAGCAGGTCAG | | TBP | TATA-box binding protein | NM_003194.4 | F: TCTTGGCGTGTGAAGATAACC | 100 | 1.87 | | R: GCTGGAACTCGTCTCACTATTC | | PPIA | Peptidylprolyl isomerase A | XM_024446809.1 | F: GGTCCCAAAGACAGCAGAAA | 115 | 1.87 | | R: GTCACCACCCTGACACATAAA | | BGLAP | Bone gamma-carboxyglutamate protein | NM_199173.5 | F: AAATAGCCCTGGCAGATTCC | 105 | 1.96 | | R: CAGCCTCCAGCACTGTTTAT | | ALPL | Alkaline phosphatase | XM_006710546.3 | F: GGAGTATGAGAGTGACGAGAAAG | 103 | — | | R: GAAGTGGGAGTGCTTGTATCT | | RUNX2 | Runt-related transcription factor 2 | NM_001278478.1 | F: TGTCATGGCGGGTAACGAT | 147 | 1.95 | | R: AAGACGGTTATGGTCAAGGTGAA | | COL1A1 | Collagen type I alpha 1 chain | XM_005257058.4 | F: GAGGGCCAAGACGAAGACATC | 140 | 1.92 | | R: CAGATCACGTCATCGCACAAC | | VDR | Vitamin D receptor | NM_001017536.1 | F: TCTCCTGCCTACTCACGATAA | 105 | — | | R: GCTACTGCCCGTGAGAATATAA | | SNAI1 | Snail family transcriptional repressor 1 | NM_005985.3 | F: CCACGAGGTGTGACTAACTATG | 126 | — | | R: ACCAAACAGGAGGCTGAAATA |
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