Safety and Localization of Mesenchymal Stromal Cells Derived from Human Adipose Tissue-Associated Hyaluronic Acid: A Preclinical Study

Figure 2

Analysis of the differentiation capacity of ASC. (a, b) Photomicrography on inverted microscope of phase contrast (magnification: 100x). (a) Negative control ASC in DMEM media and (b) differentiated ASCs presenting lipid vacuoles (magnification: 100x). (c, d) Cells stained with 1% Alizarin red, shown in (c) negative control and (d) differentiated ASCs presenting calcium deposits (magnification: 40x). (e, f) Cells stained with Alcian blue, in (e) negative control and (f) ASCs differentiated presenting the accumulation of sulfated proteoglycans (magnification: 100x).

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