Substance P Administered after Myocardial Infarction Upregulates Microphthalmia-Associated Transcription Factor, GATA4, and the Expansion of c-Kit⁺ Cells

Figure 6

SP induces the expression of MITF in c-Kit⁺ cells. (a) Western blot analysis showing the expression of targeted proteins in SP-treated c-Kit⁺ cells over time. (b) qRT-PCR analysis of relative mRNA levels of MITF in c-Kit⁺ cells after SP treatment (10 nM) at the indicated time points. or versus corresponding controls using Student’s -test. (c–e) Confocal microscopy images showing c-Kit, MITF, and GATA4 and dual staining of these targeted proteins in untreated and SP-treated c-Kit⁺ cells. DAPI (blue) allowed visualization of the nucleus in cells. Scale bars, 100 μm.

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