Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway

<div>AMSC-derived exosomes improved CPB-induced POCD in rats via the TLR2/TLR4 signaling pathway. SPF SD male rats were randomly divided into four groups including the sham operation group (sham group); CPB surgery group (CPB group); exosome+CPB group (EXO group); and exosome+CPB group+TLR2/TLR4 agonist group (TLR group) with 10 rats in each group. (a, b) TLR2, TLR4, MyD88, and NF-<i>κ</i>B expression in hippocampus tissue in each group was determined by Western blot and qRT-PCR. (c) The immunofluorescence image of TLR2, TLR4, MyD88, and NF-<i>κ</i>B in hippocampus tissue in each group (scale <span class="nowrap"><svg height="12.7373pt" id="M44" style="vertical-align:-3.429399pt" version="1.1" viewbox="-0.0498162 -9.3079 64.8 12.7373" width="64.8pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M152 404V712C115 698 54 683 7 677V654C71 648 73 642 73 579V24C128 -2 179 -12 220 -12C353 -12 471 92 471 238C471 357 381 449 274 449C262 449 249 446 233 439L152 404ZM152 374C170 384 202 393 230 393C313 393 382 326 382 213C382 97 330 26 246 26C194 26 165 62 158 81C154 91 152 101 152 116V374Z"></path></g><g transform="matrix(.013,0,0,-0.013,6.669,0)"><path d="M433 39L423 65C413 59 399 54 387 54C370 54 352 69 352 114V299C352 352 342 392 307 422C285 440 255 449 225 449C168 437 102 399 75 379C56 365 44 353 44 339C44 315 69 296 87 296C101 296 111 303 116 319C124 349 133 371 145 385C156 397 171 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Stem Cells International

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Figure 6

Figure 6: Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway 

Figure 6 | Exosomes of Antler Mesenchymal Stem Cells Improve Postoperative Cognitive Dysfunction in Cardiopulmonary Bypass Rats through Inhibiting the TLR2/TLR4 Signaling Pathway 