Antioxidant Properties of Tonsil-Derived Mesenchymal Stem Cells on Human Vocal Fold Fibroblast Exposed to Oxidative Stress

Figure 5

Dynamic changes in expression of extracellular matrix-(ECM-) related genes. (a–c) Relative mRNA expression of collagens I, III, and IV was determined to be significantly upregulated following tBHP treatment. Coculturing with TMSCs served to downregulate the expression of all collagen subtypes. (d, e) The representative immunofluorescence images for collagen staining (d). Images were quantified by measuring fluorescence intensity (e). Similar to qRT-PCR results, oxidative stress enhanced collagen expression in hVFFs while TMSC counteracted tBHP’s impact. (f) MMP activity assay results showing that decreased MMP activity in tBHP-treated hVFFs was significantly restored after coculturing with TMSCs. Results are 3 technical replicates of TMSC from one donor. Representative results from two different TMSCs with similar tendency were presented. For multigroup comparison, ANOVA followed by the Bonferroni post hoc test was used for statistics. Results are shown as the . , , and .

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