TRAF6 Promotes Gastric Cancer Cell Self-Renewal, Proliferation, and Migration

Figure 3

Knockdown of TRAF6 inhibited cell proliferation and migration in HGC-27 gastric cancer cells. (a) Real-time PCR revealed TRAF6 expression in transfected NC and TRAF6 knockdown HGC-27 cells (, ). (b) Western blot was used to confirm the expressions of TRAF6, PCNA, and LC3 in transfected NC and TRAF6 siRNA-transfected HGC-27 cells. (c) CCK8 assay for transfected NC and TRAF6 siRNA-transfected HGC-27 cells; cells were incubated at 37°C for 72 h. (d) Representative images of colony formation for transfected NC and TRAF6 siRNA-transfected HGC-27 cells; cells were cultured for 1 week. (e) Number of clones in (d) (, ). (f) Transwell migration assay for transfected NC and TRAF6 siRNA-transfected HGC-27 cells. (g) Number of migrating cells in (f) (, ).

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