hCTLA4-Gene-Modified Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMMSCs) Maintain POSTN Secretion to Enhance the Migration Capability of Allogeneic hBMMSCs through the Integrin αvβ3/FAK/ERK Signaling Pathway

Figure 6

The integrin αvβ3/FAK/ERK signaling pathway in allo-hBMMSCs was activated by the culture supernatant of CTLA4- and POSTN-modified hBMMSCs cocultured with PBMCs treated with PHA. The concentrated culture supernatant from NC hBMMSCs, CTLA4-modified hBMMSCs, POSTN-modified hBMMSCs, or CTLA4+shPOSTN-modified hBMMSCs cocultured with PBMCs treated with PHA was added into the culture medium of allo-hBMMSCs at a ratio of 1 : 10, and the treated allo-hBMMSCs were named NC, CTLA4, POSTN, and CTLA4+shPOSTN, according to their treatment. Protein levels of key factors in the integrin αvβ3/FAK/ERK signaling pathway were detected by western blotting. (a) Representative graphs of western blotting and (b) statistical results of relative protein expression based on three independent experiments. , when compared to NC group; ^#, when compared to the POSTN group.

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