hCTLA4-Gene-Modified Human Bone Marrow-Derived Mesenchymal Stem Cells (hBMMSCs) Maintain POSTN Secretion to Enhance the Migration Capability of Allogeneic hBMMSCs through the Integrin αvβ3/FAK/ERK Signaling Pathway

Figure 8

Migration capability of allo-hBMMSCs in each group was suppressed by anti-integrin αvβ3 treatment. Approximately 2 μg/mL negative control IgG or anti-integrin αvβ3 was added to the culture medium of allo-hBMMSCs with concentrated culture supernatant from CTLA4-modified hBMMSCs or POSTN-modified hBMMSCs and allo-hBMMSCs co-cultured with PBMCs treated with PHA, and the treated allo-hBMMSCs were named CTLA4+IgG, CTLA4+anti-integrin αvβ3, POSTN+IgG, or POSTN+anti-integrin αvβ3. The migration capability of allo-hBMMSCs in each group was evaluated by Transwell assay (a), wound healing assay (b), and western blotting to detect MMP2 and MMP9 levels (c). For all panels, left shows the representative images or blots and right shows the statistical results based on three independent experiments. , when compared to the IgG group.

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