Acute Myocardial Infarction Reduces Respiration in Rat Cardiac Fibers, despite Adipose Tissue Mesenchymal Stromal Cell Transplant

Table 2

Oxygen flux in respiratory experiments normalized to CS activity. Effects of AMI on the mitochondrial function of permeabilized cardiac fibers from rats after acute myocardial infarction (AMI) during the necrotic and fibrotic phases. Different parameters of the mitochondrial function of permeabilized cardiac fibers were analyzed by high-resolution respirometry, according to the multiple substrate-uncoupler-inhibitor titration protocol.

Mitochondrial function parameter (in pmol O2 s^-1 mg^-1/CS)		Necrotic phase		Fibrotic phase
	Sham group ()	BZ ()	IZ ()	BZ ()	IZ ()
CI	0.16 ± 0.008	0.18 ± 0.018	0.011 ± 0.005^#,	0.12 ± 0.024	0.009 ± 0.005^#,
CIp	1.39 ± 0.078	1.11 ± 0.104^#	0.06 ± 0.02^#,	0.55 ± 0.091^#	0.10 ± 0.026^#,
	2.7 ± 0.15	2.19 ± 0.28	0.95 ± 0.095^#,	1.31 ± 0.26^#	0.29 ± 0.06^#,
Leak state ()	1.12 ± 0.09	0.94 ± 0.125	0.97 ± 0.13	0.78 ± 0.16	0.25 ± 0.05^#,
()	1.58 ± 0.10	1.25 ± 0.18	0.05 ± 0.022^#,	0.53 ± 0.11^#
E	3.22 ± 0.16	2.48 ± 0.23^#	1.08 ± 0.13^#,	1.66 ± 0.37^#	0.32 ± 0.06^#,
CImax	1.76 ± 0.17	1.10 ± 0.16^#	0.13 ± 0.024^#,	0.64 ± 0.14^#	0.08 ± 0.022^#
CIImax	1.35 ± 0.11	1.28 ± 0.16	0.89 ± 0.14	0.97 ± 0.24	0.22 ± 0.04^#,
ROX	0.09 ± 0.008	0.10 ± 0.013	0.08 ± 0.003	0.078 ± 0.008	0.064 ± 0.012
Cytocrome C (%)	13.35 ± 1.43	14.53 ± 3	86.68 ± 26.7^#,	15.66 ± 3.03	30.59 ± 11.58

CI: nonphosphorylative state; CI_p: phosphorylative state associated with complex I; : maximal phosphorylative state; : leak state; (): flux coupled with ATP synthesis; : maximal respiratory capacity; CI_max: complex I contribution to the maximal ETS capacity; CII_max: complex II contribution to the maximal ETS capacity; ROX: residual oxygen consumption; Cyto C: an index of mitochondrial outer membrane integrity (represented as percentage). Data represent the values. ^# with respect to Sham; when comparisons are made within the 4 BZ and IZ subgroups (horizontal lines), the levels of significance are indicated by the number of asterisks: ; ; (1-way ANOVA with Bonferroni’s posttest).