High Glucose Exacerbates TNF-α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1

Figure 1

High glucose exacerbated the inhibitory effects of TNF-α on the cell viability of PDLSCs. PDLSCs were seeded on a 96-well plate and treated with 5.6 mM glucose (G5.6), 30 mM glucose (G30), 10 ng/ml TNF-α (G5.6+TNF-α), and 30 mM glucose combined with 10 ng/ml TNF-α (G30+TNF-α). (a) Morphology of PDLSCs on day 6 under a microscope. Scale bars represent 200 μm. (b) Estimated growth curve for PDLSCs using the model. (c) CCK-8 assays on day 6. The cell viability of the control group was regarded as 100%. Data are expressed as . All assays were replicated 3 times using PDLSCs obtained from 3 different individuals. versus the control group (G5.6); versus the control group (G5.6); versus the G5.6+TNF-α group.

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