High Glucose Exacerbates TNF-α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1

Figure 4

High glucose and TNF-α caused cell apoptosis in PDLSCs. PDLSCs were treated under different conditions (G5.6, G30, G5.6+TNF-α, and G30+TNF-α) for 2 days or 6 days. (a) TUNEL/DAPI double staining was used to detect apoptosis on day 2. Apoptosis cells were stained brown and indicated by red arrows. Scale bars represent 100 μm. (b) Annexin V-FITC/PI double staining was conducted to detect apoptosis on day 6. The horizontal axis represents the fluorescent intensity of annexin V-FITC, whereas the vertical axis represents the fluorescent intensity of PI. Data are expressed as . All assays were replicated 3 times using PDLSCs obtained from 3 different individuals. versus the control group (G5.6), versus the G5.6+TNF-α group.

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