High Glucose Exacerbates TNF-α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1

Figure 5

Addition of vitamin C or TNFR1 antibody totally reversed elevation of intracellular ROS expression caused by high glucose and TNF-α. PDLSCs were cultured under different conditions. Intracellular ROS level was detected by using CellROX regent and flow cytometry on day 6. (a) High glucose further increased TNF-α-induced intracellular ROS expression on day 6. Data are expressed as . All assays were replicated 3 times using PDLSCs obtained from 3 different individuals. versus the control group (G5.6), versus the G5.6+TNF-α group. (b) Vitamin C or TNFR1 antibody reverses the elevation of intracellular ROS expression on day 6. Data are expressed as . All assays were replicated 3 times using PDLSCs obtained from 3 different individuals. versus the control group (G5.6).

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