High Glucose Exacerbates TNF-α-Induced Proliferative Inhibition in Human Periodontal Ligament Stem Cells through Upregulation and Activation of TNF Receptor 1

Figure 7

Administration of vitamin C partially restored cell viability and S phase fraction in PDLSCs treated with high glucose and TNF-α. (a) Cell morphology was observed under a microscope (100x, scale bars represents 200 μm). (b) Vitamin C partially restored cell viability on day 6 (CCK-8 assay). Data are expressed as . All assays were replicated 3 times using PDLSCs obtained from 3 different individuals. versus the control group (G5.6), versus the G30+TNF-α group. (c) Vitamin C partially restored the S phase fraction on day 4 (cell cycle analysis by flow cytometry). The relative S phase fraction of the control group was regarded as 100%. Data are presented as . All assays were replicated 3 times using PDLSCs obtained from 3 different individuals. versus the control group; versus the G30+TNF-α group.

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