Modulation of Adipose-Derived Mesenchymal Stem/Stromal Cell Transcriptome by G-CSF Stimulation

Figure 2

Hierarchical cluster and Gene Ontology analysis of differentially expressed genes in ADSCs treated with G-CSF. (a) Heat map of differentially expressed genes involved in G-CSF responses in ADSCs. (b) Venn diagrams showing high consistency between the two technical replicas (R1 and R2) run for each biological triplicate for both Innovator and Biosimilar formulations (top 2 rows), and between them in terms of upregulated and downregulated genes (lower row). (c) Functional annotation showed that genes could be grouped into a limited number of biological categories. Most of the genes upregulated in ADSCs were enriched in innate immune response, differentiation, and MAPK cascade ( value < 0.001), while most of the genes downregulated are involved in cell migration and cell adhesion ( value < 0.001).

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