Research Article
Hypermethylation in Calca Promoter Inhibited ASC Osteogenic Differentiation in Rats with Type 2 Diabetic Mellitus
Table 1
Primer sequences for qPCR.
| | Gene | Forward primer sequence (5-3) | Reverse primer sequence (5-3) |
| | β-Actin | TGGCACCCAGCACAATGAA | CTAAGTCATAGTCCGCCTAGAAGCA | | RUNX2 | CCATAACGGTCTTCACAAATCCT | TCTGTCTGTGCCTTCTTGGTTC | | ALP | CCTTGTAGCCAGGCCCATTG | GGACCATTCCCACGTCTTCAC | | COL-1 | GCCTCCCAGAACATCACCTA | GCAGGGACTTCTTGAGGTTG | | OCN | CCCAGGCGCTACCTGTATCAA | GGTCAGCCAACTCGTCACAGTC | | BMP | CAACACCGTGCTCAGCTTC C | TTCCCACTCATTTCTGAAAGTTCC | | Calca | AGTCATCGCTCACCAGGGA | GGCTGCTTTCCAAGGTTGAC | | DNMT1 | TATTGCAGTCGCGGTCACTT | CTGATTGATTGGCCCCAGGT |
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Abbreviations: RUNX2: runt-related transcription factor 2; ALP: alkaline phosphatase; COL-1: collagen type I; OCN: osteocalcin; BMP: bone morphogenetic protein; Calca: calcitonin-related polypeptide; DNMT1: DNA methyltransferase 1.
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