Effect of Human Mesenchymal Stem Cells on Xenogeneic T and B Cells Isolated from Lupus-Prone MRL.Fas^lpr Mice

Figure 3

IFN-γ makes hMSCs capable of inhibiting MRL.Fas^lpr B cells. (a, b) hMSCs ( cells/well) and MRL.Fas^lpr mouse B (a) or T (b) cells ( cells/well) were cocultured for 72 h. B cells with lipopolysaccharide (LPS, 1 μg/ml) and T cells were activated with concanavalin A (ConA, 1 μg/ml). Proliferation of B and T cells was measured by the ³H-thymidine uptake assay (upper), and the levels of IgM (a) and IFN-γ (b) accumulated in culture medium were measured by ELISA (lower). (c–f) hMSCs were pretreated with IFN-γ (3–30 ng/ml) for 7 days (c, e) or with IFN-γ (10 ng/ml) for 1–7 days (d, f). hMSCs ( cells/well) and B cells ( cells/well) were cocultured for 72 h, and the levels of IgM were measured by ELISA (c, d). hMSCs ( cells/well) and T cells ( cells/well) were cocultured for 72 h, and the levels of IFN-γ accumulated in culture medium were measured by ELISA (e, f). versus control.

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