Effect of Human Mesenchymal Stem Cells on Xenogeneic T and B Cells Isolated from Lupus-Prone MRL.Fas^lpr Mice

Figure 5

IFN-hMSCs inhibit MRL.Fas^lpr B cells in a CXCL10-dependent manner. (a, b) Expression levels of chemokines in MSCs measured by RT-PCR (a) and ELISA (b). UN: untreated hMSCs. IFN: IFN-treated hMSCs. (c–e) IFN-γ-activated hMSCs (IFN-hMSCs) were transfected with negative-control, CCL2, CXCL10, or CXCL12 siRNA. Expression levels analyzed by RT-PCR (c). IFN-hMSCs ( cells/well) were added to the lower wells and MRL.Fas^lpr mouse B cells ( cells/well) to the upper wells of transwell plates with a 5 μm insert. After 1.5 h, the number of B cells migrating to the lower well was determined (d). For time-lapse imaging, IFN-hMSCs (70 μl of cells/ml) were seeded into the left chamber and B cells (70 μl of cells/ml) into the right chamber of culture-insert μ-Dish^35mm culture dishes. Images were acquired every 2 min for 12 h (). Representative photos are shown. The number of B cells passing through the white boxes is shown on the right (e). .

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