Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions

Figure 3

Suppression of multilineage differentiation capability in shCTNNB1 hESCs. (a) Immunofluorescence staining shows β-tubulin, SMA, and AFP triploblastic markers in 21-42 days differentiated hESCs (). (b) Semiquantitative RT-PCR validation of mRNA expression levels of the three germ layers in 21-42 days differentiated hESCs. The error bars denote from three independent experiments (, , , , relative to WT hESCs).

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