Essentiality of CTNNB1 in Malignant Transformation of Human Embryonic Stem Cells under Long-Term Suboptimal Conditions

Figure 5

MMC-induced malignant transformation and BFB in hESCs. (a) qRT-PCR analysis of genes expressed in 0.001 μg/ml MMC-treated cells after 10 passages. (b) Cell cycle analysis of MMC-treated cells. (c) Chromatin bridge analysis was performed in hESCs after 0.001 μg/ml MMC treatment for 48 h. Nuclei were stained with DAPI (blue) (). The total number of cells in anaphase and anaphase bridge was counted. (d) Cell growth curves of MMC-treated cells. The error bars represent from three independent experiments (, , , , relative to control hESCs).

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