RSP5 Positively Regulates the Osteogenic Differentiation of Mesenchymal Stem Cells by Activating the K63-Linked Ubiquitination of Akt

Figure 2

RSP5 expression in MSCs during osteogenic differentiation. (a) The staining of ARS became darker from day 0 to 14 of induction. The quantification of ARS staining was also increased from day 0 to 14 of induction. . (b) The staining of ALP was also darker from day 0 to 14 of induction. The ALP activity increased from day 0 to 14 of induction. . (c) The expression of RSP5 in at the protein level gradually increased during MSC osteogenic differentiation. (d) The RSP5 expression level was positively related to ARS quantification of MSCs on day 10 of induction. (e) The RSP5 expression level was positively related to the ALP activities of MSCs on day 10 of induction. indicates . . independent experiments with 3 different MSC lines.

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