RSP5 Positively Regulates the Osteogenic Differentiation of Mesenchymal Stem Cells by Activating the K63-Linked Ubiquitination of Akt

Figure 3

Inhibiting RSP5 expression decreased the osteogenic differentiation capacity of MSCs. (a) Lv-RSP5 significantly inhibited RSP5 expression in MSCs. (b) The ARS staining and quantification of the Lv-RSP5 group were weakened compared to those of the NC group. . (c) The ALP staining and activity of the Lv-RSP5 group decreased compared to those of the NC group. . (d) Runx2, OCN, and OPN expressions at the gene level were lower in the Lv-RSP5 group than the NC group. (e) The Runx2, OCN, and OPN protein expression levels were also lower in the Lv-RSP5 group than the NC group. indicates . . independent experiments with 3 different MSC lines. The induction group indicates MSCs undergoing osteogenic differentiation without other treatment. The NC group indicates MSCs transfected with control lentiviruses undergoing osteogenic differentiation. The Lv-RSP5 group indicates MSCs transfected with lentiviruses encoding an shRNA for RSP5 undergoing osteogenic differentiation.

(a)

(b)

(c)

(d)

(e)