RSP5 Positively Regulates the Osteogenic Differentiation of Mesenchymal Stem Cells by Activating the K63-Linked Ubiquitination of Akt

Figure 4

RSP5 overexpression accelerated MSC osteogenic differentiation. (a) OE-RSP5 significantly increased RSP5 expression in MSCs. (b) The ARS staining and quantification of the OE-RSP5 group were higher than those of the NC group. . (c) The ALP staining and activity of the OE-RSP5 group were also higher than those of the NC group. . (d) Runx2, OCN, and OPN expressions at the gene level increased in the OE-RSP5 group. (e) The Runx2, OCN, and OPN protein expression levels also increased in the OE-RSP5 group. indicates . . independent experiments with 3 different MSC lines. The induction group indicates MSCs undergoing osteogenic differentiation without other treatment. The NC group indicates MSCs transfected with control lentiviruses undergoing osteogenic differentiation. The OE-RSP5 group indicates MSCs transfected with lentiviruses overexpressing RSP5 undergoing osteogenic differentiation.

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