RSP5 Positively Regulates the Osteogenic Differentiation of Mesenchymal Stem Cells by Activating the K63-Linked Ubiquitination of Akt

Figure 5

RSP5 regulated the osteogenic differentiation of MSCs through the Akt signaling pathway. (a) The phosphorylated level of Akt was decreased in the Lv-RSP5 group but increased in the OE-RSP5 group. The phosphorylated levels of Smad and catenin were comparable in the Lv-RSP5 group and OE-RSP5 group. (b) OE-RSP5 increased ARS staining and quantification. AZD5363 returned these values to the normal levels in the NC group. . (c) OE-RSP5 increased ALP staining and activity. AZD5363 reversed these effects to normal levels as in the NC group. . (d) OE-RSP5 increased Runx2, OCN, and OPN expressions. AZD5363 reduced the expression of these markers to the normal levels in the NC group. indicates . . independent experiments with 3 different MSC lines. The induction group indicates MSCs undergoing osteogenic differentiation without other treatment. The NC group indicates MSCs transfected with control lentiviruses for Lv-RSP5 or OE-RSP5 undergoing osteogenic differentiation. The OE-RSP5 group indicates MSCs transfected with lentiviruses overexpressing RSP5 undergoing osteogenic differentiation.

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